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Image Search Results
Journal: Cells
Article Title: The Role of GLP1 in Rat Steatotic and Non-Steatotic Liver Transplantation from Cardiocirculatory Death Donors
doi: 10.3390/cells8121599
Figure Lengend Snippet: Effect of GLP1 on inflammatory responses in LT with DCDs. ( A ) Protein levels of suppressors of cytokine signaling 1 (SOCS1) ( A1 ), SOCS2 ( A2 ), and SOCS3 ( A3 ) in liver. ( B ) MPO ( B1 ) and MDA ( B2 ) levels in liver, Von Willebrand Factor (vWF) in plasma ( B3 ), and nitrate, nitrite ( B4 ), and nitrotyrosine ( B5 ) levels in liver. For A1–A3 and B1–B5, six transplants per group were performed for each measurement. Representative Western blots for the proteins SOCS1, SOCS2, and SOCS3 and the housekeeping protein β-actin are shown at the top, and densitometric quantification of SOCS1, SOCS2, and SOCS3 protein expression normalized to β-actin (arbitrary units) is shown at the bottom. + p < 0.05 vs. LT; # p < 0.05 vs. CD+LT.
Article Snippet: After assessing transfer, the membranes were saturated in 4 mM Tris-HCl, pH 7.6, 30 mM NaCl, and 0.1% Tween 20 (TBST) containing 5% non-fat milk and incubated overnight at 4 °C using antibodies against GLP1 (ab23468), cyclin A (sc-239), CD-26 (also known as DPP4; sc-52642), SOCS3 (sc-9023) and transferrin (sc-52256) (Santa Cruz Biotechnology, Dallas, TX, USA), SOCS1 (ARP42148) and
Techniques: Clinical Proteomics, Western Blot, Expressing